Colorectal cancer cell lines show striking diversity of their O-glycome reflecting the cellular differentiation phenotype.

ABSTRACT: Alterations in protein glycosylation in colorectal cancer (CRC) have been extensively studied using cell lines as models. However, little is known about their O-glycome and the differences in glycan biosynthesis in different cell types. To provide a better understanding of the variation in O-glycosylation phenotypes and their association with other molecular features, an in-depth O-glycosylation analysis of 26 different CRC cell lines was performed. The released O-glycans were analysed on porous graphitized carbon nano-liquid chromatography system coupled to a mass spectrometer via electrospray ionization (PGC-nano-LC-ESI-MS/MS) allowing isomeric separation as well as in-depth structural characterization. Associations between the observed glycan phenotypes with previously reported cell lin

SUBMITTER: Madunic K 

PROVIDER: S-EPMC7867528 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature