Platelet electrical resistance for measuring platelet activation and adhesion in human health and disease.

ABSTRACT:

Background

The ability to measure changes in platelet reactivity is important to identify novel aspects of platelet biology and develop targeted therapeutics to prevent bleeding or thrombosis. Current platelet function testing allows for single agonist analysis at a time. The ability to phenotype platelets in a single assay with multiple agonists and adhesion substrates could yield more insights into altered pathways than are feasible with current approaches. We hypothesized platelet electrical resistance (PER) could be used for more comprehensive phenotyping of platelets.

Methods

Platelets were isolated from male and female healthy donors (age 39.6 ± 6.9) and septic patients (age 44.0 ± 13.5). PER 96-well plates were coated with various substrates, including fibrinogen and

SUBMITTER: Griffiths TM 

PROVIDER: S-EPMC7867577 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature