RPB5-mediating protein promotes the progression of non-small cell lung cancer by regulating the proliferation and invasion.

ABSTRACT:

Background

This study aimed to investigate the relationship between RNA polymerase II subunit 5 (RPB5)-mediating protein (RMP) and clinicopathological characteristics of non-small cell lung cancer (NSCLC) patients by measuring the expression level of RMP in human NSCLC tissues and cell lines. At the same time, we studied the impact of RMP on the biological function of cancer, providing strong support for gene targeted therapy of NSCLC.

Methods

Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot were used to determine the expression levels of messenger (m)RNA and protein in NSCLC cell lines and tissues. Cell counting kit 8 (CCK8) assay and flow cytometry were selected to detect cell proliferation, cycle and apoptosis. The wound he

SUBMITTER: Feng Y 

PROVIDER: S-EPMC7867794 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature