Immune profiling and immunotherapeutic targets in pancreatic cancer.

ABSTRACT:

Background

Immunotherapeutic approaches for pancreatic ductal adenocarcinoma (PDAC) are less successful as compared to many other tumor types. In this study, comprehensive immune profiling was performed in order to identify novel, potentially actionable targets for immunotherapy.

Methods

Formalin-fixed paraffin embedded (FFPE) specimens from 68 patients were evaluated for expression of 395 immune-related markers (RNA-seq), mutational burden by complete exon sequencing of 409 genes, PD-L1 expression by immunohistochemistry (IHC), pattern of tumor infiltrating lymphocytes (TILs) infiltration by CD8 IHC, and PD-L1/L2 copy number by fluorescent in situ hybridization (FISH).

Results

The seven classes of actionable genes capturing myeloid immunosuppression, metabolic immuno

SUBMITTER: Lenzo FL 

PROVIDER: S-EPMC7867882 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature