Structural insight and stability of TNFR-Fc fusion protein (Etanercept) produced by using transgenic silkworms.

ABSTRACT: Therapeutic proteins expressed using transgenic animals have been of great interest for several years. Especially, transgenic silkworm has been studied intensively because of its ease in handling, low-cost, high-yield and unique glycosylation patterns. However, the physicochemical property of the therapeutic protein expressed in transgenic silkworm remains elusive. Here, we constructed an expression system for the TNFR-Fc fusion protein (Etanercept) using transgenic silkworm. The TNFR-Fc fusion protein was employed to N-glycan analysis, which revealed an increased amount of afucosylated protein. Evidence from surface plasmon resonance analysis showed that the TNFR-Fc fusion protein exhibit increased binding affinity for Fcγ receptor IIIa and FcRn compared to the commercial Etanercept, emph

SUBMITTER: Kiyoshi M 

PROVIDER: S-EPMC7868081 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature