In vivo imaging of a PVD neuron in Caenorhabditis elegans.

ABSTRACT: The nematode Caenorhabditis elegans nociceptive PVD neurons have highly ordered dendritic branches, making this an ideal model to study the development and organization of dendrites. A ser-2-promoter-driven GFP reporter line wyIs592[ser-2prom-3p::myr-GFP] provides a comprehensive visualization of PVD anatomy. Here, we describe the detailed procedures for imaging a PVD neuron using wyIs592 at late L4 larval stage in vivo by confocal microscopy. This protocol can also be applied to imaging other cells in C. elegans. For complete details on the use and execution of this protocol, please refer to Feng et al. (2020).

SUBMITTER: Wang X 

PROVIDER: S-EPMC7868634 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature