MiRNome profiling of LSC-enriched CD34⁺CD38^-CD26⁺ fraction in Ph⁺ CML-CP samples from Argentinean patients: a potential new pharmacogenomic tool.

ABSTRACT: Chronic myeloid leukemia (CML) is a myeloid stem cell neoplasm characterized by an expansion of myeloid progenitor cells and the presence of BCR-ABL1 oncoprotein. Since the introduction of specific BCR-ABL1 tyrosine kinase inhibitors (TKI), overall survival has improved significantly. However, under long-term therapy patients may have residual disease that originates from TKI-resistant leukemic stem cells (LSC). In this work, we analyzed the miRNome of LSC-enriched CD34⁺CD38^-CD26⁺ and normal hematopoietic stem cells (HSC) fractions obtained from the same chronic phase (CP) CML patients, and stem and progenitor cells obtained from healthy donors (HD) by next-generation sequencing. We detected a global decrease of microRNA levels in LSC-enriched CD34⁺

SUBMITTER: Ruiz MS 

PROVIDER: S-EPMC7869017 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature