Multiplex recording of cellular events over time on CRISPR biological tape.

ABSTRACT: Although dynamics underlie many biological processes, our ability to robustly and accurately profile time-varying biological signals and regulatory programs remains limited. Here we describe a framework for storing temporal biological information directly in the genomes of a cell population. We developed a "biological tape recorder" in which biological signals trigger intracellular DNA production that is then recorded by the CRISPR-Cas adaptation system. This approach enables stable recording over multiple days and accurate reconstruction of temporal and lineage information by sequencing CRISPR arrays. We further demonstrate a multiplexing strategy to simultaneously record the temporal availability of three metabolites (copper, trehalose, and fucose) in the environment of a cell population

SUBMITTER: Sheth RU 

PROVIDER: S-EPMC7869111 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature