Molecular characterization and expression analysis of annexin B3 and B38 as secretory proteins in Echinococcus granulosus.

ABSTRACT:

Background

Cystic echinococcosis is a parasitic zoonotic disease, which poses a threat to public health and animal husbandry, and causes significant economic losses. Annexins are a family of phospholipid-binding proteins with calcium ion-binding activity, which have many functions.

Methods

Two annexin protein family genes [Echinococcus granulosus annexin B3 (EgAnxB3) and EgAnxB38] were cloned and molecularly characterized using bioinformatic analysis. The immunoreactivity of recombinant EgAnxB3 (rEgAnxB3) and rEgAnxB38 was investigated using western blotting. The distribution of EgAnxB3 and EgAnxB38 in protoscoleces (PSCs), the germinal layer, 18-day strobilated worms and 45-day adult worms was analyzed by immunofluorescence localization, and their secretory characteristics

SUBMITTER: Song H 

PROVIDER: S-EPMC7869467 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature