Chromatin interaction analyses elucidate the roles of PRC2-bound silencers in mouse development.

ABSTRACT: Lineage-specific gene expression is modulated by a balance between transcriptional activation and repression during animal development. Knowledge about enhancer-centered transcriptional activation has advanced considerably, but silencers and their roles in normal development remain poorly understood. Here, we performed chromatin interaction analyses of Polycomb repressive complex 2 (PRC2), a key inducer of transcriptional gene silencing, to uncover silencers, their molecular identity and associated chromatin connectivity. Systematic analysis of cis-regulatory silencer elements reveals their chromatin features and gene-targeting specificity. Deletion of certain PRC2-bound silencers in mice results in transcriptional derepression of their interacting genes and pleiotropic developmental pheno

SUBMITTER: Ngan CY 

PROVIDER: S-EPMC7869692 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature