Electrochemiluminescence aptasensor for Siglec-5 detection based on MoS₂@Au nanocomposites emitter and exonuclease III-powered DNA walker.

ABSTRACT: Lectins are highly specific binding proteins for glycoproteins which widely exist in living organisms, playing a vital role in exploring the biological evolution process, such as cellular proliferation, differentiation, carcinogenesis and apoptosis. Therefore, the content monitoring of lectin becomes particularly significant and urgent in the bioanalytical application. In this work, we fabricated an aptasensor, majorly capitalizing the eminent affinity between sialic acid-binding immunoglobulin (Ig)-like lectin 5 (Siglec-5) and nucleic acids aptamer (K19), with nontoxic MoS₂@Au nanocomposites as electrochemiluminescence (ECL) emitters based on exonuclease III (Exo III)-powered DNA walker for the bioassays of Siglec-5. The DNA track was constructed on the emitters' surface, provi

SUBMITTER: Fan Z 

PROVIDER: S-EPMC7869706 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature