RCRnorm: An integrated system of random-coefficient hierarchical regression models for normalizing NanoString nCounter data.

ABSTRACT: Formalin-fixed paraffin-embedded (FFPE) samples have great potential for biomarker discovery, retrospective studies, and diagnosis or prognosis of diseases. Their application, however, is hindered by the unsatisfactory performance of traditional gene expression profiling techniques on damaged RNAs. NanoString nCounter platform is well suited for profiling of FFPE samples and measures gene expression with high sensitivity, which may greatly facilitate realization of scientific and clinical values of FFPE samples. However, methodological development for normalization, a critical step when analyzing this type of data, is far behind. Existing methods designed for the platform use information from different types of internal controls separately and rely on an overly-simplified assumption that e

SUBMITTER: Jia G 

PROVIDER: S-EPMC7869841 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature