Image-Based Elastography of Heterochromatin and Euchromatin Domains in the Deforming Cell Nucleus.

ABSTRACT: Chromatin of the eukaryotic cell nucleus comprises microscopically dense heterochromatin and loose euchromatin domains, each with distinct transcriptional ability and roles in cellular mechanotransduction. While recent methods are developed to characterize the mechanics of nucleus, measurement of intranuclear mechanics remains largely unknown. Here, the development of "nuclear elastography," which combines microscopic imaging and computational modeling to quantify the relative elasticity of the heterochromatin and euchromatin domains, is described. Using contracting murine embryonic cardiomyocytes, nuclear elastography reveals that the heterochromatin is almost four times stiffer than the euchromatin at peak deformation. The relative elasticity between the two domains changes rapidly durin

SUBMITTER: Ghosh S 

PROVIDER: S-EPMC7869959 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature