Compensation of physiological motion enables high-yield whole-cell recording in vivo.

ABSTRACT:

Background

Whole-cell patch-clamp recording in vivo is the gold-standard method for measuring subthreshold electrophysiology from single cells during behavioural tasks, sensory stimulations, and optogenetic manipulation. However, these recordings require a tight, gigaohm resistance, seal between a glass pipette electrode's aperture and a cell's membrane. These seals are difficult to form, especially in vivo, in part because of a strong dependence on the distance between the pipette aperture and cell membrane.

New method

We elucidate and utilize this dependency to develop an autonomous method for placement and synchronization of pipette's tip aperture to the membrane of a nearby, moving neuron, which enables high-yield seal formation and subsequent recordings deep in the brai

SUBMITTER: Stoy WM 

PROVIDER: S-EPMC7869963 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature