Postsynaptic Targeting and Mobility of Membrane Surface-Localized hASIC1a.

ABSTRACT: Acid-sensing ion channels (ASICs), the main H⁺ receptors in the central nervous system, sense extracellular pH fluctuations and mediate cation influx. ASIC1a, the major subunit responsible for acid-activated current, is widely expressed in brain neurons, where it plays pivotal roles in diverse functions including synaptic transmission and plasticity. However, the underlying molecular mechanisms for these functions remain mysterious. Using extracellular epitope tagging and a novel antibody recognizing the hASIC1a ectodomain, we examined the membrane targeting and dynamic trafficking of hASIC1a in cultured cortical neurons. Surface hASIC1a was distributed throughout somata and dendrites, clustered in spine heads, and co-localized with postsynaptic markers. By extracellular pHluori

SUBMITTER: Song XL 

PROVIDER: S-EPMC7870742 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature