Microclusters as T Cell Signaling Hubs: Structure, Kinetics, and Regulation.

ABSTRACT: When T cell receptors (TCRs) engage with stimulatory ligands, one of the first microscopically visible events is the formation of microclusters at the site of T cell activation. Since the discovery of these structures almost 20 years ago, they have been studied extensively in live cells using confocal and total internal reflection fluorescence (TIRF) microscopy. However, due to limits in image resolution and acquisition speed, the spatial relationships of signaling components within microclusters, the kinetics of their assembly and disassembly, and the role of vesicular trafficking in microcluster formation and maintenance were not finely characterized. In this review, we will summarize how new microscopy techniques have revealed novel insights into the assembly of these structures. The su

SUBMITTER: Balagopalan L 

PROVIDER: S-EPMC7870797 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature