Cross-linking mass spectrometry uncovers protein interactions and functional assemblies in synaptic vesicle membranes.

ABSTRACT: Synaptic vesicles are storage organelles for neurotransmitters. They pass through a trafficking cycle and fuse with the pre-synaptic membrane when an action potential arrives at the nerve terminal. While molecular components and biophysical parameters of synaptic vesicles have been determined, our knowledge on the protein interactions in their membranes is limited. Here, we apply cross-linking mass spectrometry to study interactions of synaptic vesicle proteins in an unbiased approach without the need for specific antibodies or detergent-solubilisation. Our large-scale analysis delivers a protein network of vesicle sub-populations and functional assemblies including an active and an inactive conformation of the vesicular ATPase complex as well as non-conventional arrangements of the lumina

SUBMITTER: Wittig S 

PROVIDER: S-EPMC7870876 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature