Deletion of Mir223 Exacerbates Lupus Nephritis by Targeting S1pr1 in Fas^lpr/lpr Mice.

ABSTRACT:

Objective

The micro RNAs (miRNAs) and their target mRNAs are differentially expressed in various immune-mediated cells. Here, we investigated the role of Mir223 and sphingosine-1-phosphate receptor 1 (S1pr1) in the pathogenesis of systemic lupus erythematosus.

Methods

We analyzed miRNA and mRNA profiling data of CD4⁺ splenic T cells derived from MRL/MpJ-Fas^lpr /J mice. We performed 3' untranslated region (UTR) luciferase reporter gene assay using human umbilical vein endothelial cells (HUVECs). We generated the B6-Mir223^-/-Fas^lpr/lpr mice and the lupus phenotypes were analyzed.

Results

In CD4⁺ splenic T cells, we identified upregulation of miR-223-3p and downregulation of the pos

SUBMITTER: Hiramatsu-Asano S 

PROVIDER: S-EPMC7871001 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature