Direct conversion of adult human retinal pigmented epithelium cells to neurons with photoreceptor properties.

ABSTRACT: Degeneration of photoreceptors is a major cause of blindness. Identifying new methods for the generation of photoreceptors offers valuable options for a cell replacement therapy of blindness. Here, we show that primary adult human retinal pigmented epithelium (hRPE) cells were directly converted to postmitotic neurons with various properties of photoreceptors by the neurogenic transcription factor ASCL1 and microRNA124. At Day 8 after the induction of ASCL1 and miRNA124 expression in hRPE cells, 91% of all cells were Tuj1⁺, and 83% of all cells were MAP2⁺ neurons. The cone photoreceptor marker L/M-opsin, the rod photoreceptor marker rhodopsin, and the generic photoreceptor marker recoverin were expressed in 76%, 86%, and 92% of all cells, respectively. Real-time quant

SUBMITTER: Li B 

PROVIDER: S-EPMC7871113 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature