Multilabel Per-Pixel Quantitation in Mass Spectrometry Imaging.

ABSTRACT: In quantitative mass spectrometry imaging (MSI), the gold standard adds a single structural homologue of the target compound at a known concentration to the sample. This internal standard enables to map the detected intensity of the target molecule against an external calibration curve. This approach, however, ignores local noise levels and disproportional ion suppression effects, which might depend on the concentration of the target compound. To overcome these issues, we propose a novel approach that applies several isotopically labeled versions, each at a different concentration, to the sample. This allows creating individual internal calibration curves for every MSI pixel. As proof of principle, we have quantified an endogenous peptide of histone H4 by matrix-assisted laser desorption/i

SUBMITTER: Dewez F 

PROVIDER: S-EPMC7871324 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature