High-Throughput Fractionation Coupled to Mass Spectrometry for Improved Quantitation in Metabolomics.

ABSTRACT: Metabolomics is emerging as an important field in life sciences. However, a weakness of current mass spectrometry (MS) based metabolomics platforms is the time-consuming analysis and the occurrence of severe matrix effects in complex mixtures. To overcome this problem, we have developed an automated and fast fractionation module coupled online to MS. The fractionation is realized by the implementation of three consecutive high performance solid-phase extraction columns consisting of a reversed phase, mixed-mode anion exchange, and mixed-mode cation exchange sorbent chemistry. The different chemistries resulted in an efficient interaction with a wide range of metabolites based on polarity, charge, and allocation of important matrix interferences like salts and phospholipids. The use of shor

SUBMITTER: van der Laan T 

PROVIDER: S-EPMC7871441 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature