ImmunoPET imaging of human CD8⁺ T cells with novel ⁶⁸Ga-labeled nanobody companion diagnostic agents.

ABSTRACT:

Background

Although immunotherapy has revolutionized treatment strategies for some types of cancers, most patients failed to respond or obtain long-term benefit. Tumor-infiltrating CD8⁺ T lymphocytes are closely related to the treatment outcome and prognosis of patients. Therefore, noninvasive elucidation of both systemic and tumor-infiltrating CD8⁺ T lymphocytes is of extraordinary significance for patients during cancer immunotherapy. Herein, a panel of ⁶⁸Ga-labeled Nanobodies were designed and investigated to track human CD8⁺ T cells in vivo through immuno-positron emission tomography (immunoPET).

Results

Among the screened Nanobodies, SNA006a showed the highest binding affinity and specificity to both human CD8 protein and CD8<

SUBMITTER: Zhao H 

PROVIDER: S-EPMC7871532 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature