Comparison of neurofilament light chain results between two independent facilities.

ABSTRACT:

Objectives

To examine levels of neurofilament light chain (NFL) in identical cerebrospinal fluid (CSF) and blood samples at two different facilities, and how differences affect interpretation of levels within and above the normal range.

Methods

CSF and plasma from patients with multiple sclerosis (MS) and healthy controls (HCs) were analysed by Simoa (Quanterix) for levels of NFL providing a total of 165 CSF samples (119 from MS) and 225 plasma samples (180 from MS).

Results

CSF and plasma concentrations highly correlated between NFL laboratory facilities (R: 0.92 and 0.84, <0.0001, respectively), and there were no differences between facilities. A bias between the two sites for plasma was -0.95 pg/mL and for CSF -73.53 pg/mL. The cut-offs for CSF were 807.5 and 571.0

SUBMITTER: Sejbaek T 

PROVIDER: S-EPMC7871722 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature