Analysis of β-d-glucan biosynthetic genes in oat reveals glucan synthesis regulation by light.

ABSTRACT:

Background and aims

Oat (Avena sativa) has human health benefits when consumed as a whole-grain food, attributed to the high content of (1,3;1,4)-β-d-glucan (mixed-linkage glucan [MLG]), but little is known about the synthase genes and synthesis mechanism of MLG polysaccharides in this species.

Methods

The concentration of oat MLGs under different light intensities was measured by a standard enzymatic approach and further verified by immunoelectron microscopy. The effect of light intensity on MLG synthase genes was examined by RT-qPCR and western blot analyses. The pattern of expression directed by the promoter of the oat MLG synthase gene was also investigated by histochemical β-glucuronidase (GUS) analysis.

Key results

The oat orthologues of genes implicated in the

SUBMITTER: Zhang J 

PROVIDER: S-EPMC7872105 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature