Automated phenotyping and lifespan assessment of a C. elegans model of Parkinson's disease.

ABSTRACT: Phenotypic analysis of Caenorhabditis elegans has greatly advanced our understanding of the molecular mechanisms implicated in the aging process as well as in age-related pathologies. However, conventional high-resolution imaging methods and survival assays are labor-intensive and subject to operator-based variations and decreased reproducibility. Recent advances in microfluidics and automated flatbed scanner technologies have significantly improved experimentation by eliminating handling errors and increasing the sensitivity in measurements. Here, we introduce a medium-throughput microfluidic platform, which efficiently positions and immobilizes single worms through pressurization for high resolution imaging. Worms are sorted based on select imaging criteria, and subsequently trans

SUBMITTER: Kim M 

PROVIDER: S-EPMC7872154 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature