Cardiomyocyte Na⁺/H⁺ Exchanger-1 Activity Is Reduced in Hypoxia.

ABSTRACT: Fully-activated Na⁺/H⁺ exchanger-1 (NHE1) generates the cardiomyocyte's largest trans-membrane extrusion of H⁺ ions for an equimolar influx of Na⁺ ions. This has the desirable effect of clearing excess intracellular acidity, but comes at a large energetic premium because the exchanged Na⁺ ions must ultimately be extruded by the sodium pump, a process that consumes the majority of the heart's non-contractile ATP. We hypothesize that the state of NHE1 activation depends on metabolic resources, which become limiting in periods of myocardial hypoxia. To test this functionally, NHE1 activity was measured in response to in vitro and in vivo hypoxic treatments. NHE1 flux was interrogated as a function of intracellular pH by fluo

SUBMITTER: Kandilci HB 

PROVIDER: S-EPMC7873356 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature