Proteome Composition of Bovine Amniotic Membrane and Its Potential Role in Corneal Healing.

ABSTRACT:

Purpose

To investigate the protein profile of bovine amniotic membranes (bAM) and to determine putative associations between protein composition in bAM and known corneal healing pathways.

Methods

The bAM were acquired from normal full-term births (n = 10), processed, and stored at -80°C for two days. Subsequently, the frozen membranes were thawed at room temperature and prepared for proteomic exploration using high-resolution liquid chromatography-mass spectrometry, followed by bioinformatics analysis. Recently identified corneal healing pathways were contrasted with protein profiles and pathways present in bAM.

Results

The analyses identified 2105 proteins, with an interactive network of 1271 nodes (proteins) and 8757 edges (interactions). The proteins with higher be

SUBMITTER: Capistrano da Silva E 

PROVIDER: S-EPMC7873491 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature