Rapid Degradation Pathways of Host Proteins During HCMV Infection Revealed by Quantitative Proteomics.

ABSTRACT: Human cytomegalovirus (HCMV) is an important pathogen in immunocompromised individuals and neonates, and a paradigm for viral immune evasion. We previously developed a quantitative proteomic approach that identified 133 proteins degraded during the early phase of HCMV infection, including known and novel antiviral factors. The majority were rescued from degradation by MG132, which is known to inhibit lysosomal cathepsins in addition to the proteasome. Global definition of the precise mechanisms of host protein degradation is important both to improve our understanding of viral biology, and to inform novel antiviral therapeutic strategies. We therefore developed and optimized a multiplexed comparative proteomic analysis using the selective proteasome inhibitor bortezomib in addition to MG13

SUBMITTER: Lin KM 

PROVIDER: S-EPMC7873559 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature