A tandem motif-based and structural approach can identify hidden functional phosphodiesterases.

ABSTRACT: Cyclic nucleotide monophosphates (cNMPs) are increasingly recognized as essential signaling molecules governing many physiological and developmental processes in prokaryotes and eukaryotes. Degradation of cNMPs is as important as their generation because it offers the capability for transient and dynamic cellular level regulation but unlike their generating enzymes, the degrading enzymes, cyclic nucleotide phosphodiesterases (PDEs) are somewhat elusive in higher plants. Based on sequence analysis and structural properties of canonical PDE catalytic centers, we have developed a consensus sequence search motif and used it to identify candidate PDEs. One of these is an Arabidopsis thaliana K⁺-Uptake Permease (AtKUP5). Structural and molecular docking analysis revealed that t

SUBMITTER: Kwiatkowski M 

PROVIDER: S-EPMC7873575 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature