Atypical B cells are part of an alternative lineage of B cells that participates in responses to vaccination and infection in humans.

ABSTRACT: The diversity of circulating human B cells is unknown. We use single-cell RNA sequencing (RNA-seq) to examine the diversity of both antigen-specific and total B cells in healthy subjects and malaria-exposed individuals. This reveals two B cell lineages: a classical lineage of activated and resting memory B cells and an alternative lineage, which includes previously described atypical B cells. Although atypical B cells have previously been associated with disease states, the alternative lineage is common in healthy controls, as well as malaria-exposed individuals. We further track Plasmodium-specific B cells after malaria vaccination in naive volunteers. We find that alternative lineage cells are primed after the initial immunization and respond to booster doses. However, alternative lineag

SUBMITTER: Sutton HJ 

PROVIDER: S-EPMC7873835 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature