Single-Cell Analysis Reveals Characterization of Infiltrating T Cells in Moderately Differentiated Colorectal Cancer.

ABSTRACT:

Objective

This study aimed to characterize the tumor-infiltrating T cells in moderately differentiated colorectal cancer.

Methods

Using single-cell RNA sequencing data of isolated 1632 T cells from tumor tissue and 1252 T cells from the peripheral blood of CRC patients, unsupervised clustering analysis was performed to identify functionally distinct T cell populations, followed by correlations and ligand-receptor interactions across cell types. Finally, differential analysis of the tumor-infiltrating T cells between colon cancer and rectal cancer were carried out.

Results

A total of eight distinct T cell populations were identified from tumor tissue. Tumor-Treg showed a strong correlation with Th17 cells. CD8⁺T_RM was positively correlated with CD

SUBMITTER: Yang X 

PROVIDER: S-EPMC7873865 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature