Dataset Information

Tissue-engineered autologous peritoneal grafts for bladder reconstruction in a porcine model.

ABSTRACT: Ileal neobladder construction is a common treatment for patients with bladder cancer after radical cystectomy. However, metabolic disorders caused by transposed bowel segments occur frequently. Bladder tissue engineering is a promising alternative approach. Although numerous studies have reported bladder reconstruction using acellular and cellular scaffolds, there are also disadvantages associated with these methods, such as immunogenicity of synthetic grafts and incompatible mechanical properties of the biomaterials. Here, we engineered an autologous peritoneal graft consisting of a peritoneal sheet and the seromuscular layer from the ileum. Three months after the surgery, compared with the neobladder made from the ileum, the reconstructed neobladder using our new method showed normal function and better gross morphological characteristics. Moreover, histopathological and transcriptomic analysis revealed urothelium-like cells expressing urothelial biomarkers appeared in the neobladder, while no such changes were observed in the control group. Overall, our study provides a new strategy for bladder tissue engineering and informs a variety of future research prospects.

SUBMITTER: Chen B

PROVIDER: S-EPMC7874343 | biostudies-literature | 2021 Jan-Dec

REPOSITORIES: biostudies-literature

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Tissue-engineered autologous peritoneal grafts for bladder reconstruction in a porcine model.

Chen Biao B Chen Xia X Wang Wenjia W Shen Jun J Song Zhiqiang Z Ji Haoyu H Zhang Fangyuan F Wu Jianchen J Na Jie J Li Shengwen S

Journal of tissue engineering 20210101

Ileal neobladder construction is a common treatment for patients with bladder cancer after radical cystectomy. However, metabolic disorders caused by transposed bowel segments occur frequently. Bladder tissue engineering is a promising alternative approach. Although numerous studies have reported bladder reconstruction using acellular and cellular scaffolds, there are also disadvantages associated with these methods, such as immunogenicity of synthetic grafts and incompatible mechanical properti ...[more]

PMID: 33613958

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Project description:IntroductionSurgical replacement of dysfunctional cardiac muscle with regenerative tissue is an important option to combat heart failure. But current available myocardial prostheses like a Dacron or a pericardium patch neither have a regenerative capacity nor do they actively contribute to the heart`s pump function. This study aimed to show the feasibility of utilizing a vascularized stomach patch for transmural left ventricular wall reconstruction.MethodsA left ventricular transmural myocardial defect was reconstructed by performing transdiaphragmatic autologous transplantation of a vascularized stomach segment in six Lewe minipigs. Three further animals received a conventional Dacron patch as a control treatment. The first three animals were followed up for 3 months until planned euthanasia, whereas the observation period for the remaining three animals was scheduled 6 months following surgery. Functional assessment of the grafts was carried out via cardiac magnetic resonance tomography (MRI) and angiography. Physiological remodeling was evaluated histologically and immunohistochemically after heart explanation.ResultsFive out of six test animals and all control animals survived the complex surgery and completed the follow-up without clinical complications. One animal died intraoperatively due to excessive bleeding. No animal experienced a rupture of the stomach graft. Functional integration of the heterotopically transplanted stomach into the surrounding myocardium was observed. Angiography showed the development of connections between the gastric graft vasculature and the coronary system of the host cardiac tissue.ConclusionsThe clinical results and the observed physiological integration of gastric grafts into the cardiac structure demonstrate the feasibility of vascularized stomach tissue as a myocardial prosthesis. The physiological remodeling indicates a regenerative potential of the graft. Above all, the connection of the gastric vessels with the coronary system constitutes a rationale for the use of vascularized and therefore viable stomach tissue for versatile tissue engineering applications.

Dataset Information

Tissue-engineered autologous peritoneal grafts for bladder reconstruction in a porcine model.

Publications

Tissue-engineered autologous peritoneal grafts for bladder reconstruction in a porcine model.

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