BK Channels Regulate LPS-induced CCL-2 Release from Human Pulmonary Endothelial Cells.

ABSTRACT: We recently established a role for the stretch-activated two-pore-domain K⁺ (K2P) channel TREK-1 (K2P2.1) in inflammatory cytokine secretion using models of hyperoxia-, mechanical stretch-, and TNF-α-induced acute lung injury. We have now discovered the expression of large conductance, Ca²⁺-activated K⁺ (BK) channels in human pulmonary microvascular endothelial cells and primary human alveolar epithelial cells using semiquantitative real-time PCR, IP and Western blot, and investigated their role in inflammatory cytokine secretion using an LPS-induced acute lung injury model. As expected, LPS induced IL-6 and CCL-2 secretion from pulmonary endothelial and epithelial cells. BK activation with NS1619 decreased LPS-induced CCL-2 but not IL-6 secretion from endo

SUBMITTER: Zyrianova T 

PROVIDER: S-EPMC7874395 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature