Elevated MST1 leads to apoptosis via depletion of YAP1 in cardiomyocytes exposed to high glucose.

ABSTRACT:

Background

Gestational diabetes mellitus is a risk factor for congenital heart defects. The article aimed to investigate the expression and roles of MST1, YAP1, Last1/2 and Survivin in modulating HG-induced cardiomyocyte apoptosis and maternal diabetes-induced heart abnormality.

Methods

Diabetes mellitus was induced in rats using streptozotocin. The protein expression and phosphorylation analysis in fetal heart tissue was assessed by western blot and immunohistochemical staining. Hoechst 33342 staining assay was performed to explore H9C2 apoptosis. The gene and protein expression in H9C2 cells was assessed by quantitative PCR and western blot. Knockdown of gene expression was assessed by RNA interference.

Results

Our results revealed that increased MST1 protein levels

SUBMITTER: Su D 

PROVIDER: S-EPMC7874454 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature