RIP-seq reveals LINE-1 ORF1p association with p-body enriched mRNAs.

ABSTRACT:

Background

Long INterspersed Element-1 (LINE-1) is an autonomous retroelement able to "copy-and-paste" itself into new loci of the host genome through a process called retrotransposition. The LINE-1 bicistronic mRNA codes for two proteins, ORF1p, a nucleic acid chaperone, and ORF2p, a protein with endonuclease and reverse transcriptase activity. Both proteins bind LINE-1 mRNA in cis and are necessary for retrotransposition. While LINE-1 transcription is usually repressed in most healthy somatic cells through a plethora of mechanisms, ORF1p expression has been observed in nearly 50% of tumors, and new LINE-1 insertions have been documented in a similar fraction of tumors, including prostate cancer.

Results

Here, we utilized RNA ImmunoPrecipitation (RIP) and the L1EM analysis

SUBMITTER: Briggs EM 

PROVIDER: S-EPMC7874467 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature