5'-tRF-GlyGCC: a tRNA-derived small RNA as a novel biomarker for colorectal cancer diagnosis.

ABSTRACT:

Background

tRNA-derived small RNAs (tDRs), which are widely distributed in human tissues including blood and urine, play an important role in the progression of cancer. However, the expression of tDRs in colorectal cancer (CRC) plasma and their potential diagnostic values have not been systematically explored.

Methods

The expression profiles of tDRs in plasma of CRC and health controls (HCs) are investigated by small RNA sequencing. The level and diagnostic value of 5'-tRF-GlyGCC are evaluated by quantitative PCR in plasma samples from 105 CRC patients and 90 HCs. The mechanisms responsible for biogenesis of 5'-tRF-GlyGCC are checked by in vitro and in vivo models.

Results

5'-tRF-GlyGCC is dramatically increased in plasma of CRC patients compared to that of HCs. The a

SUBMITTER: Wu Y 

PROVIDER: S-EPMC7874477 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature