CRISPRi screens reveal genes modulating yeast growth in lignocellulose hydrolysate.

ABSTRACT:

Background

Baker's yeast is a widely used eukaryotic cell factory, producing a diverse range of compounds including biofuels and fine chemicals. The use of lignocellulose as feedstock offers the opportunity to run these processes in an environmentally sustainable way. However, the required hydrolysis pretreatment of lignocellulosic material releases toxic compounds that hamper yeast growth and consequently productivity.

Results

Here, we employ CRISPR interference in S. cerevisiae to identify genes modulating fermentative growth in plant hydrolysate and in presence of lignocellulosic toxins. We find that at least one-third of hydrolysate-associated gene functions are explained by effects of known toxic compounds, such as the decreased growth of YAP1 or HAA1, or increased grow

SUBMITTER: Gutmann F 

PROVIDER: S-EPMC7874482 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature