Low-fat/high-fibre diet prehabilitation improves anastomotic healing via the microbiome: an experimental model.

ABSTRACT:

Background

Both obesity and the presence of collagenolytic bacterial strains (Enterococcus faecalis) can increase the risk of anastomotic leak. The aim of this study was to determine whether mice chronically fed a high-fat Western-type diet (WD) develop anastomotic leak in association with altered microbiota, and whether this can be mitigated by a short course of standard chow diet (SD; low fat/high fibre) before surgery.

Methods

Male C57BL/6 mice were assigned to either SD or an obesogenic WD for 6 weeks followed by preoperative antibiotics and colonic anastomosis. Microbiota were analysed longitudinally after operation and correlated with healing using an established anastomotic healing score. In reiterative experiments, mice fed a WD for 6 weeks were exposed to a SD for 2

SUBMITTER: Hyoju SK 

PROVIDER: S-EPMC7875206 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature