Pulse-Controlled Amplification-A new powerful tool for on-site diagnostics under resource limited conditions.

ABSTRACT:

Background

Molecular diagnostics has become essential in the identification of many infectious and neglected diseases, and the detection of nucleic acids often serves as the gold standard technique for most infectious agents. However, established techniques like polymerase chain reaction (PCR) are time-consuming laboratory-bound techniques while rapid tests such as Lateral Flow Immunochromatographic tests often lack the required sensitivity and/or specificity.

Methods/principle findings

Here we present an affordable, highly mobile alternative method for the rapid identification of infectious agents using pulse-controlled amplification (PCA). PCA is a next generation nucleic acid amplification technology that uses rapid energy pulses to heat microcyclers (micro-scale metal he

SUBMITTER: Muller K 

PROVIDER: S-EPMC7875409 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature