Knockout of MYOM1 in human cardiomyocytes leads to myocardial atrophy via impairing calcium homeostasis.

ABSTRACT: Myomesin-1 (encoded by MYOM1 gene) is expressed in almost all cross-striated muscles, whose family (together with myomesin-2 and myomesin-3) helps to cross-link adjacent myosin to form the M-line in myofibrils. However, little is known about its biological function, causal relationship and mechanisms underlying the MYOM1-related myopathies (especially in the heart). Regrettably, there is no MYMO1 knockout model for its study so far. A better and further understanding of MYOM1 biology is urgently needed. Here, we used CRISPR/Cas9 gene-editing technology to establish an MYOM1 knockout human embryonic stem cell line (MYOM1^-/- hESC), which was then differentiated into myomesin-1 deficient cardiomyocytes (MYOM1^-/- hESC-CMs) in vitro. We found that myomesin-1 plays an impor

SUBMITTER: Hang C 

PROVIDER: S-EPMC7875908 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature