A non-invasive method to generate induced pluripotent stem cells from primate urine.

ABSTRACT: Comparing the molecular and cellular properties among primates is crucial to better understand human evolution and biology. However, it is difficult or ethically impossible to collect matched tissues from many primates, especially during development. An alternative is to model different cell types and their development using induced pluripotent stem cells (iPSCs). These can be generated from many tissue sources, but non-invasive sampling would decisively broaden the spectrum of non-human primates that can be investigated. Here, we report the generation of primate iPSCs from urine samples. We first validate and optimize the procedure using human urine samples and show that suspension- Sendai Virus transduction of reprogramming factors into urinary cells efficiently generates integration-fre

SUBMITTER: Geuder J 

PROVIDER: S-EPMC7876031 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature