Synergistic Roles of Macrophages and Neutrophils in Osteoarthritis Progression.

ABSTRACT:

Objective

To evaluate the role of immune cells and their effector cytokines in the pathogenesis and progression of knee osteoarthritis (OA) in matched OA synovial fluid (SF) and synovial tissue samples.

Methods

Cells from matched samples of synovial tissue and SF acquired from individuals undergoing total knee replacement for OA (n = 39) were characterized for immune cell-associated surface markers and intracellular cytokine expression using polychromatic flow cytometry. Additional individuals with radiographic knee OA (Kellgren/Lawrence severity grades ≥1) who had available etarfolatide (inflammatory cell) imaging (n = 26) or baseline and 3-year data on progression of radiographic knee OA (n = 85) were also assessed. SF cytokine concentrations in all cohorts were evaluated

SUBMITTER: Hsueh MF 

PROVIDER: S-EPMC7876152 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature