The Small GTPase Arf6 Functions as a Membrane Tether in a Chemically-Defined Reconstitution System.

ABSTRACT: Arf-family small GTPases are essential protein components for membrane trafficking in all eukaryotic endomembrane systems, particularly during the formation of membrane-bound, coat protein complex-coated transport carriers. In addition to their roles in the transport carrier formation, a number of Arf-family GTPases have been reported to physically associate with coiled-coil tethering proteins and multisubunit tethering complexes, which are responsible for membrane tethering, a process of the initial contact between transport carriers and their target subcellular compartments. Nevertheless, whether and how indeed Arf GTPases are involved in the tethering process remain unclear. Here, using a chemically-defined reconstitution approach with purified proteins of two representative Arf isoform

SUBMITTER: Fujibayashi K 

PROVIDER: S-EPMC7876375 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature