Identification and Characterisation of Putative Enhancer Elements in Mouse Embryonic Stem Cells.

ABSTRACT: Enhancer elements control mammalian transcription largely in a cell-type-specific manner. The genome-wide identification of enhancer elements and their activity status in a cellular context is therefore fundamental to understanding cell identity and function. We determined enhancer activity in mouse embryonic stem (ES) cells using chromatin modifications and characterised their global properties. Specifically, we first grouped enhancers into 5 groups using multiple H3K4me1, H3K27ac, and H3K27me3 modification data sets. Active enhancers (simultaneous presence of H3K4me1 and H3K27ac) were enriched for binding of pluripotency factors and were found near pluripotency-related genes. Although both H3K4me1-only and active enhancers were enriched for super-enhancers and a TATA box like motif, acti

SUBMITTER: Mantsoki A 

PROVIDER: S-EPMC7876754 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature