LINC00649 promotes bladder cancer malignant progression by regulating the miR‑15a‑5p/HMGA1 axis.

ABSTRACT: The aim of the present study was to explore the effects of LINC00649 on the proliferation, migration and invasion of bladder cancer (BC) and identify possible mechanisms. Through TCGA database analysis of LINC00649 expression in bladder cancer and the association of LINC00649 with the BC patient prognosis, RT‑qPCR was employed for detecting LINC00649 expression in 60 clinical tissue specimens and cell lines of bladder cancer. The lentivirus stable transfection or small interfering RNA was used to increase or decrease the LINC00649 expression level in T24 and UM‑UC‑3 cells. CCK8 and clone formation assay were utilized to observe the effects of LINC00649 on the proliferation and colony formation of BC cells. Transwell experiment was performed to detect the effects of LINC00649 on the migrati

SUBMITTER: Chen X 

PROVIDER: S-EPMC7876990 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature