CD44 activation state regulated by the CD44v10 isoform determines breast cancer proliferation.

ABSTRACT: The cell surface glycoprotein CD44 displays different active statuses; however, it remains unknown whether the activation process of CD44 is critical for tumor development and progression. The aim of the present study was to investigate whether breast cancer (BCa) cells with different activation states of CD44 show similar or distinct functional characteristics and to further examine the mechanisms regulating CD44 activities. A feature for the 'activated' state of CD44 is that it can bind to its principal ligand hyaluronan (HA). The binding of CD44 with HA is usually influenced by CD44 alternative splicing, resulting in multiple CD44 isoforms that determine CD44 activities. Flow cytometry was used to sort BCa cell subsets based on CD44‑HA binding abilities (HA^‑/low vs. HA^hi

SUBMITTER: Guo Q 

PROVIDER: S-EPMC7876991 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature