P2X-GCaMPs as Versatile Tools for Imaging Extracellular ATP Signaling.

ABSTRACT: ATP is an extracellular signaling molecule involved in numerous physiological and pathologic processes. However, in situ characterization of the spatiotemporal dynamic of extracellular ATP is still challenging because of the lack of sensor with appropriate specificity, sensitivity, and kinetics. Here, we report the development of biosensors based on the fusion of cation permeable ATP receptors (P2X) to genetically encoded calcium sensors [genetically encoded calcium indicator (GECI)]. By combining the features of P2X receptors with the high signal-to-noise ratio of GECIs, we generated ultrasensitive green and red fluorescent sniffers that detect nanomolar ATP concentrations in situ and also enable the tracking of P2X receptor activity. We provide the proof of concept that these sensors can

SUBMITTER: Ollivier M 

PROVIDER: S-EPMC7877454 | biostudies-literature | 2021 Jan-Feb

REPOSITORIES: biostudies-literature