Luminescent Surfaces with Tailored Angular Emission for Compact Dark-Field Imaging Devices.

ABSTRACT: Dark-field microscopy is a standard imaging technique widely employed in biology that provides high image contrast for a broad range of unstained specimens¹. Unlike bright-field microscopy, it accentuates high spatial frequencies and can therefore be used to emphasize and resolve small features. However, the use of dark-field microscopy for reliable analysis of blood cells, bacteria, algae, and other marine organisms often requires specialized, bulky microscope systems, and expensive additional components, such as dark-field-compatible objectives or condensers^2,3. Here, we propose to simplify and downsize dark-field microscopy equipment by generating the high-angle illumination cone required for dark field microscopy directly within the sample substrate. We introduce

SUBMITTER: Chazot CAC 

PROVIDER: S-EPMC7877670 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature