Integrated spatial genomics reveals global architecture of single nuclei.

ABSTRACT: Identifying the relationships between chromosome structures, nuclear bodies, chromatin states and gene expression is an overarching goal of nuclear-organization studies^1-4. Because individual cells appear to be highly variable at all these levels⁵, it is essential to map different modalities in the same cells. Here we report the imaging of 3,660 chromosomal loci in single mouse embryonic stem (ES) cells using DNA seqFISH+, along with 17 chromatin marks and subnuclear structures by sequential immunofluorescence and the expression profile of 70 RNAs. Many loci were invariably associated with immunofluorescence marks in single mouse ES cells. These loci form 'fixed points' in the nuclear organizations of single cells and often appear on the surfaces of nuclear bodies and

SUBMITTER: Takei Y 

PROVIDER: S-EPMC7878433 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature