Supercritical carbon dioxide decellularization of plant material to generate 3D biocompatible scaffolds.

ABSTRACT: The use of plant-based biomaterials for tissue engineering has recently generated interest as plant decellularization produces biocompatible scaffolds which can be repopulated with human cells. The predominant approach for vegetal decellularization remains serial chemical processing. However, this technique is time-consuming and requires harsh compounds which damage the resulting scaffolds. The current study presents an alternative solution using supercritical carbon dioxide (scCO₂). Protocols testing various solvents were assessed and results found that scCO₂ in combination with 2% peracetic acid decellularized plant material in less than 4 h, while preserving plant microarchitecture and branching vascular network. The biophysical and biochemical cues of the scCO

SUBMITTER: Harris AF 

PROVIDER: S-EPMC7878742 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature